Snapgene confocal microscopy8/27/2023 ![]() ![]() The purpose of the Nikon DEES system is to increase the efficiency of light diffraction by the gratings used to separate fluorescence emission into component wavelengths.Įxplore real-time confocal microscopy of integrated circuits by adjusting focus depth.ĭiscover how individual fluorophores can be identified within a complex mixture. Nikon Diffraction Efficiency Enhancement System (DEES).The lambda stack is a three-dimensional dataset that consists of an image collection using the same specimen field acquired at different wavelength bands, each spanning a limited spectral region ranging from 2 to 20 nanometers. Resonant scanning mirrors generate the speed for real-time imaging of living cells.Ī virtual microscope tutorial featuring a wide variety of specimens. Resonant Scanning in Laser Confocal Microscopy.Laser alignment, photobleaching, probes, magnification, and other important concepts.Ĭonfocal microscopy can be used to produce images of triple-labeled fluorescent samples. Critical Aspects of Confocal Microscopy.The investigation of opaque specimens can be coupled with standard techniques.įixation, permeabilization, and staining with immunofluorescence and synthetic dyes. How the trade-offs involved in objective design can affect confocal microscopy.ĭistinguishing individual spectral profiles of fluorophores within complex mixtures. Optical sectioning, live-cell imaging, and time-lapse sequence acquisition. A discussion of point scanning and pinhole detection using photomultipliers. ![]()
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